⚠ Research Use Only: All content is for qualified researchers conducting in-vitro laboratory studies. Not for human consumption or veterinary use.
BAC Water vs Sterile Water: Which to Use?
| Factor | BAC Water | Sterile Water |
|---|---|---|
| Shelf life (reconstituted) | 28 days | 24–48 hours |
| Bacterial protection | Yes (0.9% benzyl alcohol) | No |
| Multiple uses | Yes | Single use recommended |
| Best for | Multi-day protocols | Single-dose research |
Recommendation: For most research applications, bacteriostatic water is the preferred choice due to its extended stability and antimicrobial protection.
Essential Equipment
- Lyophilized peptide vial
- Bacteriostatic water (BAC water)
- Sterile syringes: 1mL, 3mL, or 5mL
- Needles: 18–22 gauge for drawing, 27–30 gauge for injection into vial
- Alcohol swabs (70% isopropyl)
- Sharps disposal container
- Permanent marker for labeling
Step-by-Step Reconstitution Protocol
- Calculate volume: Volume (mL) = Peptide Amount (mg) ÷ Desired Concentration (mg/mL). Example: 5mg vial + 2.5mL BAC water = 2mg/mL.
- Prepare workspace: Wash hands, clean surface with 70% IPA, wear gloves.
- Prepare vial: Remove plastic cap, wipe rubber stopper with alcohol swab, let air dry 30 seconds.
- Draw diluent: Use 18–22 gauge needle to draw calculated BAC water volume, then switch to 27–30 gauge needle.
- Add diluent (critical): Insert needle at 45° angle, inject slowly DOWN THE SIDE of the vial wall — never directly onto the powder.
- Dissolve: Swirl gently in circular motions. Let sit 2–5 minutes. The solution should be clear. Never shake.
- Label and store: Write peptide name, concentration, reconstitution date, and expiry. Refrigerate immediately at 2–8°C.
⚠ Critical: NEVER shake the vial. Shaking breaks peptide bonds and can reduce potency by 20–50%. Use only gentle swirling motions.
Peptide-Specific Notes
Retatrutide (5mg vial)
Add 2.5mL BAC water for 2mg/mL. Takes 3–5 minutes to fully dissolve. Solution should be clear.
Semaglutide (5mg vial)
Add 2.5mL BAC water for 2mg/mL. Very stable — dissolves quickly in 1–2 minutes.
Melanotan 2 / MT2 (10mg vial)
Add 2mL BAC water for 5mg/mL. Highly photosensitive — wrap in aluminum foil immediately. Protect from light at all times.
Common Mistakes to Avoid
- Injecting directly onto powder → creates foam, denatures peptides. Always inject down the vial wall.
- Shaking the vial → breaks peptide bonds. Only gentle swirling.
- Using non-sterile water → contamination risk. Only pharmaceutical-grade BAC water or SWFI.
- Freezing reconstituted peptides → ice crystals damage peptide structure. Refrigerate only.
- Forgetting to label → always mark concentration and date immediately.
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All PeptideMax peptides are ≥98% pure, lyophilized, and include full COA documentation.
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